Inflammatory markers increase the risk of cardiovascular disease. Predicting the risk of coronary artery disease, serves an important role in treating and preventing the progression of the disease. Aerobic exercise decreases the risk of coronary artery disease by reducing inflammation. The current study investigated the effect of a period of selective aerobic exercise on the serum levels of CRP, fibrinogen, and IL-6 among the middle-aged women. Twenty-four middle-aged women were equally split into two groups, control and experimental. The experimental group underwent 30-min exercise sessions with the increasing intensity of 50-75% maximum heart rate, three times a week for 8 weeks. Blood samples were taken from both groups twice (24 hours before and 24 hours after the training) in order for the measurement of the intended factors. A paired sample t-test was used to compare the pre-test and post-test data. In order to test the difference between the two groups, independent t-test was used at a significant level (P<05/0). Normality of the data was checked by using the Kolmogorov Smirnov test. The results of the study revealed that 8 weeks of aerobic exercise induced a significant difference in the levels of CRP (p = 0.001), fibrinogen (p = 0.004), and IL-6 (p = 0.022) between the two groups. According to current research results, performing a period of regular aerobic exercise, while applying the overload principle and intensity, can cause the reduction of inflammatory markers of cardiovascular disease, which along with aging can be a determining factor in the atherosclerosis, especially in sedentary people