In this study, a novel fructose-based DES of choline chloride (2-hydroxyethyl-trimethylammonium) has been synthesized at different molar ratios. The physical properties such as density, viscosity, surface tension, refractive index and pH were measured and analyzed as function of various temperatures (25–85 °C). The analysis of these physical properties revealed that these new DESs have the potential to be utilized for possible industrial applications involving processing and separation of food constituents. The suggested DESs have many desirable characteristics, e.g. they have low vapor pressure, inflammable, biodegradable, and made from renewable resources. The use of these DESs will positively affect the environment and make use of available renewable resources